Journal: European Journal of Medical Research

Article Title: A case of solitary plasmacytoma of bone showing co-expression of both immunoglobulin light chains

doi: 10.1186/s40001-021-00621-8

Figure Lengend Snippet: Flow cytometry of the tumor revealed that CD38-positive cells co-expressed kappa and lambda light chain. Fluorescein isothiocyanate (FITC)-labeled CD38 antibody (T16, Beckman Coulter, Inc., CA), FITC-labeled cytoplasmic kappa light chain antibody [Rabbit Polyclonal F(ab′)2, Agilent Technologies, CA], and phycoerythrin (PE)-labeled cytoplasmic lambda light chain antibody [Rabbit Polyclonal F(ab′)2, Agilent Technologies, CA] were used for the flow cytometry

Article Snippet: Fluorescein isothiocyanate (FITC)-labeled CD38 antibody (T16, Beckman Coulter, Inc., CA), FITC-labeled cytoplasmic kappa light chain antibody [Rabbit Polyclonal F(ab′)2, Agilent Technologies, CA], and phycoerythrin (PE)-labeled cytoplasmic lambda light chain antibody [Rabbit Polyclonal F(ab′)2, Agilent Technologies, CA] were used for the flow cytometry Fig. 3 Tumor biopsy of the neck (sampled at 2019). a H&E staining (×400) shows marked plasma cells infiltration. b CD138 staining (×200) shows diffuse positivity for these plasma cells. c , d In situ hybridization of kappa and lambda light chain mRNA (×200) and e , f immunohistochemistry of kappa and lambda light chain (×200) We reviewed the previous specimen and performed additional IgL immunohistochemistry.

Techniques: Flow Cytometry, Labeling

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: The B-cell stimulatory cytokines BLyS and APRIL are elevated in human periodontitis and are required for B-cell–dependent bone loss in experimental murine periodontitis 1

doi: 10.4049/jimmunol.1500496

Figure Lengend Snippet: (A) Immunohistochemical analysis of human tonsil shows diffuse staining of kappa light chain (green) within the connective tissue indicating the presence of B cells/plasma cells (red arrows). (B) Immunohistochemical analysis of human gingival tissue from a patient with chronic periodontitis shows prominent staining of kappa light chain (green) within the connective tissue adjacent to the basement membrane (red arrow). In both the tonsil (A) and the gingiva (B), occasional kappa-light-chain–positive cells infiltrate the superficial epithelial layer (yellow arrows). (C) Control staining of human gingival tissue from a patient with chronic periodontitis using FITC-conjugated non-immune IgG. Nuclear staining (blue) by DAPI. E, epithelium; CT, connective tissue. Original magnification 100X. Scale bars, 100 μm.

Article Snippet: To this end, the following Abs were used: goat anti-human APRIL polyclonal IgG (Abcam), goat anti-human BLyS polyclonal IgG (R&D Systems), and FITC-conjugated rabbit anti-human Ig kappa light chain polyclonal IgG (DakoCytomation) that recognizes B cells and Ig-secreting plasma cells ( 30 ).

Techniques: Immunohistochemical staining, Staining